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Two antigens fromMycobacterium tuberculosis , ESAT-6 and MPT64, elicited delayed-type hypersensitivity (DTH) skin responses in outbred guinea pigs infected withM. tuberculosis by the aerosol and intravenous routes but not those sensitized withM. bovis BCG orM. avium . The DTH epitope of ESAT-6 was mapped to the C terminus. Nonresponders to the individual antigens were found, but all animals responded to a combination of ESAT-6 and MPT64 or their respective minimal target peptides. Correspondingly, these molecules could form the basis of a new skin test for tuberculosis.

Delayed-Type Hypersensitivity Responses to ESAT-6 and MPT64 fromMycobacterium tuberculosisin the Guinea Pig