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Release of Shiga toxin fromShigella dysenteriae 1 was found to occur after exposure to polymyxin B. The amount of toxin released was dependent on both the polymyxin concentration and time of incubation. An immunoblot characterization of the Shiga toxin released by polymyxin treatment demonstrated that it is electrophoretically similar to purified Shiga toxin and to Shiga toxin present in crude bacterial sonicates ofS. dysenteriae 1 cells.

Release of Shiga Toxin from Shigella dysenteriae 1 by Polymyxin B