Open AccessDraft Genome Sequence of Marine Cyanobacterium Synechococcus sp. Strain NKBG042902, Which Harbors a Homogeneous Plasmid Available for Metabolic Engineering
Author(s) -
Toru Honda,
Liang Yue,
Daichi Arai,
Yasuhito Ito,
Tomoko Yoshino,
Tsuyoshi Tanaka
Publication year - 2014
Publication title -
genome announcements
Language(s) - English
Resource type - Journals
ISSN - 2169-8287
DOI - 10.1128/genomea.00704-14
Subject(s) - plasmid , strain (injury) , contig , biology , whole genome sequencing , escherichia coli , genome , metabolic engineering , homogeneous , gene , genetics , physics , anatomy , thermodynamics
The marine cyanobacterium Synechococcus sp. strain NKBG042902 was isolated from coastal areas in Japan. Strain NKBG042902 has four plasmids: pSY8, pSY9, pSY10, and pSY11. Moreover, the hybrid plasmid pUSY02 containing pSY11 and Escherichia coli plasmid pUC18 was constructed for this strain. The genetic manipulation technique using pUSY02 was established for this strain and used in metabolic engineering. Here, we report the draft genome sequence of this strain, which has 77 contigs comprising a total length of 3,319,479 bp, with a G+C content of 49.4%.