Production of Trichothecene Mycotoxins by Fusarium Species in Shake Culture

Twelve T-2 toxin-producing isolates and four fusarenon-X-producing isolates ofFusarium species were examined for their ability to produce trichothecene mycotoxins in shake culture and jar fermentation. T-2 toxin producers such asFusarium solani, F. sporotrichioides , andF. tricinctum produced T-2 toxin and neosolaniol in semisynthetic medium.F. solani M-1-1 produced the largest amount of the mycotoxins in a nutrient medium consisting of 5% glucose (or sucrose), 0.1% peptone, and 0.1% yeast extract in either shake culture or jar fermentation at 24 to 27 C for 5 days. None of the isolates produced significant amounts of fusarenon-X in shake cultures.