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Pseudomonas SB15, which produces extracellular isoamylase, was found to produce intracellular α-glucosidase and amylase(s) when grown on maltose. A mutant strain (MS1) derived from it, which formed isoamylase constitutively, also produced these intracellular enzymes constitutively. The activities of the enzymes produced in the mutant strain were much greater than those induced in the parent strain.

Formations of Extracellular Isoamylase and Intracellular α-Glucosidase and Amylase(s) by Pseudomonas SB15 and a Mutant Strain