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A rapid slide agglutination test has been developed for the identification ofNeisseria gonorrhoeae that are primarily detected as oxidase-positive colonies in gonococcal cultures. The technique is based on the specific nonimmune reactivity between the Fc portion of immunoglobulin (Ig)G and staphylococcal protein A. IgG molecules adsorbed to stabilized staphylococci will thereby become oriented with their antigen-reactive sites that are directed outwards. Protein A-containing staphylococci with unabsorbed anti-gonococcal antibodies gave positive co-agglutination reactions with gonococci but also with meningococci, someMoraxella, Haemophilus , andPseudomonas strains. These crossreactions were eliminated by absorption of the anti-gonococcal antiserum with meningococcal andMoraxella organisms prior to the coating of reagent staphylococci. In the routine culture diagnosis ofN. gonorrhoeae the use of specific gonococcal reagent staphylococci gave concordant results with fermentation procedures and immunofluorescent techniques.

applied microbiology

Slide Agglutination Method for the Serological Identification of <i>Neisseria gonorrhoeae</i> with Anti-Gonococcal Antibodies Adsorbed to Protein A-Containing Staphylococci

Slide Agglutination Method for the Serological Identification of Neisseria gonorrhoeae with Anti-Gonococcal Antibodies Adsorbed to Protein A-Containing Staphylococci