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A bacteriphage typing scheme for differentiatingProteus isolated from clinical specimens was developed. Twenty-one distinct patterns of lysis were seen when 15 bacteriophages isolated on 8Proteus mirabilis , 1P. vulgaris , and 1P. morganii were used to type 162 of 189 (85.7%)P. mirabilis andP. vulgaris isolates. Seven phages isolated on 3P. morganii were used to type 13 of 19 (68.4%)P. morganii isolates. Overall, 84.1% of the 208 isolates were lysed by at least 1 phage at routine test dilution (RTD) or 1,000 × RTD. Fifty isolates, retyped several weeks after the initial testing, showed no changes in lytic patterns. The phages retained their titers after storage at 4 C for several months. A computer analysis of the data showed that there was no relationship between the source of the isolate and bacteriophage type. This bacteriophage typing system may provide epidemiological information on strains involved in human infections.

Bacteriophage Typing of Proteus mirabilis, Proteus vulgaris , and Proteus morganii