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A microtissue culture method for the plaque assay ofHerpesvirus saimiri has been developed. Virus titrations carried out in Microtest II tissue culture plates (Falcon) yielded reproducible results that agreed well with those obtained by employing macrocultures. The described method is quantitative, reproducible, economical, and suitable for routine assay of large numbers of virus samples.

Microtissue Culture Plaque Assay for Herpesvirus saimiri