Open AccessDirect Fluorescent-Antibody Technique for the Microbiological Examination of Food and Environmental Swab Samples for Salmonellae
Author(s) -
N. F. Insalata,
C. W. Mahnke,
W. G. Dunlap
Publication year - 1973
Publication title -
applied microbiology
Language(s) - English
Resource type - Journals
ISSN - 0003-6919
DOI - 10.1128/am.26.3.268-270.1973
Subject(s) - false positive paradox , salmonella , fluorescence , chromatography , food science , biology , microbiology and biotechnology , chemistry , bacteria , mathematics , statistics , genetics , physics , quantum mechanics
Comparative studies of a modified fluorescent-antibody procedure and the 5 to 7 day method used by the Association of Official Analytical Chemists for the detection ofSalmonella were made on 151 samples of wheat products and 183 swab samples. The agreement between the two methods for the 334 samples tested was 92.5%. Food samples yielded 94.7% agreement, whereas the swab samples yielded 90.7% agreement. There were 7.5% false positives for the total number of samples tested. No false negatives were obtained by using the fluorescent-antibody method.
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