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Insoluble polyelectrolytes (PE60) were used for the concentration of viruses from stool specimens, confirming the results of Wallis et al. (1969). Ten percent suspensions inoculated with poliovirus type 3 were used in these experiments. A small number of stool specimens from patients naturally infected with enteroviruses were also tested. Preferential adsorption of viruses to PE60 was maximum at ap H range of 4.5 to 6.0. The elution of the adsorbed viruses was optimal atp H 8.5. Other parameters were also investigated. Electron microscopy was used successfully to detect the eluted viruses.

Use of Polyelectrolytes and Electron Microscopy for Detection of Viruses from Stool