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The speciation of 262 strains of group D streptococci isolated from human sources is described. One hundred forty-two isolates from blood cultures were included; 96 of these were submitted as isolates from clinical cases of subacute bacterial endocarditis. The results show that 98Streptococcus faecalis , 29S. faecalis var.zymogenes , 44S. faecalis var.liquefaciens , 27S. faecium , 13S. durans , 44S. bovis , and 7 unspeciatedS. bovis -like group D isolates were identified. NoS. faecium var.casseliflavus, S. equinus , orS. avium (group Q streptococci) were identified among the human isolates. The speciation procedures and techniques are detailed. The procedures and limitations of the tests used are discussed. Ninety-eight percent of the 262 strains were speciated by a spectrum of tests that allowed us to recognize atypical as well as typical strains within species.

Recognition of Group D Streptococcal Species of Human Origin by Biochemical and Physiological Tests