Open AccessHemagglutination Inhibition for Serogrouping of Neisseria meningitidis
Author(s) -
Richard L. Cohen,
Malcolm S. Artenstein
Publication year - 1972
Publication title -
applied microbiology
Language(s) - English
Resource type - Journals
ISSN - 0003-6919
DOI - 10.1128/am.23.2.289-292.1972
Subject(s) - neisseria meningitidis , microbiology and biotechnology , hemagglutination , antiserum , agglutination (biology) , direct agglutination test , hemagglutination assay , antigen , virology , serotype , biology , neisseria , bacteria , chemistry , antibody , serology , immunology , virus , genetics , titer
A hemagglutination inhibition (HI) test has been studied as an alternative to bacterial agglutination (BA) for serogrouping strains ofNeisseria meningitidis isolated from clinical specimens. The HI test consists of polysaccharide antigens adsorbed to sheep red blood cells which were then agglutinated by group-specific antisera. Supernatant fluids from suspensions of meningococci were used to inhibit the agglutination. Results of the two tests agreed for 381 (80%) carrier strains. Of the remaining 95 strains, 82 (86%) were identified by HI although they were nongroupable by BA. Thus, the HI test has been shown to be more highly specific and sensitive and to be more economical of reagents and time than the BA test.
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