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A method has been developed to produce and purify gram quantities of T-2 toxin [4β, 15-diacetoxy-8α-(3-methylbutyryloxy)-12, 13-epoxytrichothec-9-en-3α-ol], a mycotoxin elaborated by a strain ofFusarium tricinctum isolated from toxic corn. After growing for 3 weeks at 15 C on 1,200 g of white corn grits,F. tricinctum NRRL 3299 elaborated at least 9.0 g of T-2 toxin, and 2.3 g of crystalline product was recovered. A lesser amount of toxin was produced on rice, but none was detected in wheat incubated at 20 C. The amount of toxin measured in white corn grits declined as the incubation temperature was raised to 20, 25, and 32 C.

T-2 Toxin Production by Fusarium tricinctum on Solid Substrate