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Suspensions of influenza virus were dried by sublimation of ice in vacuo to contents of residual moisture of 2.8, 1.6, or 0.6%. After drying, the preparations were sealed under helium, argon, nitrogen, or a mixture of oxygen and nitrogen (30% O2 , 70% N2 ). Stabilities of the several preparations were determined by an accelerated storage test. Based on the times predicted for the dried preparations stored at preselected temperatures to lose 1 log of infectivity titer, the order of stabilities in relation to residual moisture, gas was as follows: 1.6%, He &gt; 0.6%, He &gt; 2.8%, He &gt; 1.6%, Ar &gt; 2.8%, N2 &gt; 2.8%, Ar &gt; 1.6%, N2 &gt; 2.8%, O2 &gt; 0.6%, Ar &gt; 0.6%, N2 &gt; 1.6%, O2 &gt; 0.6%, O2 . The stability of the preparation sealed under helium and dried to the content of residual moisture found best for this gas (1.6%) resulted in an increased stability of the order of years as compared to the other preparations tested.

applied microbiology

Stabilities of Suspensions of Influenza Virus Dried by Sublimation of Ice In Vacuo to Different Contents of Residual Moisture and Sealed Under Different Gases