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A schedule was developed for the simultaneous production of aPasteurella tularensis-Brucella abortus antiserum in rabbits. Three doses of 108 viableP. tularensis LVS organisms were given intravenously at weekly intervals. One day prior to the final dose ofP. tularensis , the rabbits received 109 viable cells ofB. abortus strain 19 intravenously. The use of live vaccines, administered in this sequence, resulted in high agglutinin titers within 3 weeks. The maximal agglutinin titer to either organism was observed 1 week after the final injection.

Agglutinin Response of Rabbits to Combined Pasteurella tularensis-Brucella abortus Vaccination