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The effects of NH4 Cl concentration, organic nitrogen compounds, glucose concentration, dissolved oxygen concentration, and flow rate on the attached growth of pure cultures ofSphaerotilus natans and of a mixed population in a continuous-flow apparatus are described. Low concentrations of NH4 Cl and oxygen, and high flow rates resulted in attached populations that were dominated bySphaerotilus . The conditions that allowed maximal attached growth in pure culture did not correspond to the conditions that promoted attached growth ofSphaerotilus in a mixed population.

Attached Growth of Sphaerotilus and Mixed Populations in a Continuous-flow Apparatus