Open AccessEffect of Ion Concentration on the Rupture and Survival of Azotobacter Cysts
Author(s) -
Millicent C. Goldschmidt,
Orville Wyss
Publication year - 1968
Publication title -
applied microbiology
Language(s) - English
Resource type - Journals
ISSN - 0003-6919
DOI - 10.1128/am.16.6.871-876.1968
Subject(s) - ethylenediaminetetraacetic acid , azotobacter , phosphate , dipicolinic acid , germination , chemistry , chelation , tonicity , salt (chemistry) , nuclear chemistry , inorganic chemistry , microbiology and biotechnology , biochemistry , biology , bacteria , spore , agronomy , organic chemistry , genetics
The ability of ethylenediaminetetraacetic acid (EDTA) and dipicolinic acid (DPA) to ruptureAzotobacter cysts was directly related to the molarity of the reaction mixture. Cysts were not ruptured at concentrations above 0.15m (NaCl or phosphate). These higher salt concentrations prevented the formation of metallo:EDTA (or DPA) chelate complexes. Sudden shifts in the tonicity of NaCl or phosphate mixtures induced cyst rupture in the absence of EDTA. The effect of these mechanisms on the survival and germination ofAzotobacter cysts in the soil is discussed.
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