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The sporicidal activity of peracetic acid (PAA) vapor at 20, 40, 60, and 80% relative humidity (RH) and 25 C was determined onBacillus subtilis var.niger spores on paper and glass surfaces. Appreciable activity occurred within 10 min of exposure to 1 mg of PAA per liter and 40% or higher RH. The sporicidal rate decreased from the optimum at 80% RH to a slight effect at 20% RH. Spores on an impermeable surface were more difficult to kill than those on a porous one, probably because the cells tend to pile up on an impermeable surface and the vapor penetrates poorly through the layer of covering cells.

Sporicidal Effect of Peracetic Acid Vapor