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Cultivation ofNeisseria gonorrhoeae was effected in a conical glass culture vessel surrounded by a constant-temperature water jacket, and with facilities for stirring, aeration, andp H measurement and control. With the use of an aerated peptone-based medium, containing polypropylene glycol to prevent foam build-up, the yields obtained over thep H range from 5.8 to 7.4 were determined. The greatest yield was obtained atp H 6.4 when the dry weight was 1.5 g/liter. Atp H 7.2 to 7.6, lysis was extensive.

Dense Cultures of Neisseria gonorrhoeae in Liquid Medium