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Samples of sugar, starch, spices, and miscellaneous products were tested for thermophilic sporeformers ofBacillus to determine the dominant species present. Surface colonies selected at random were identified. Six species ofBacillus were isolated:B. stearothermophilus, B. coagulans, B. licheniformis, B. subtilis, B. circulans , andB. pumilus . Samples of starch and pepper were tested for thermophilic sporeformers ofBacillus to determine the distribution of rough and smooth variants. Colonies were classified as rough or smooth variants by colonial characteristics. The distribution of variant forms in these two products was significantly different. Starch samples showed predominantly rough variants; pepper samples showed predominantly smooth variants.

Distribution of Thermophilic Aerobic Sporeforming Bacteria in Food Ingredients