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Bench-scale studies withChlorella pyrenoidosa 7-11-05 were conducted in a 4-liter culture vessel with a used and recycled medium. Algal cultures were maintained for periods of several weeks by supplementing the nutrient medium with minimal amounts of certain salts. An algal strain was maintained for a period of up to 72 days with a supplemented recycled medium. No inhibition was observed as the result of any autotoxic materials. Rather dense cultures were maintained in the presence of high bacterial populations.

Growth of Chlorella pyrenoidosa in Recycled Medium