Production Model Press for the Preparation of Bacterial Cell Walls
Author(s) -
Theodore D. Perrine,
E. Ribi,
Wusi C. Maki,
Brittney Miller,
E. Oertli
Publication year - 1962
Publication title -
applied microbiology
Language(s) - English
Resource type - Journals
ISSN - 0003-6919
DOI - 10.1128/am.10.2.93-98.1962
Subject(s) - yield (engineering) , alloy , materials science , corrosion , chemistry , chemical engineering , composite material , engineering
A modification of the apparatus previously described permits the preparation of cell walls in quantity. This consists of a heavy duty, double-acting hydraulic press with motor-driven pump, and a superstrength alloy steel pressure cell which is corrosion resistant. Liquid cooling of the jet is substituted for the previously used gas cooling to minimize aerosol formation and to facilitate subsequent treatment of the products. The device produces cell walls of excellent quality in good yield. The pressure cell has been used satisfactorily up to about 60,000 psi. Design details are given.
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