Open AccessDevelopment of 16S rRNA-Based Probes for the Coriobacterium Group and the Atopobium Cluster and Their Application for Enumeration of Coriobacteriaceae in Human Feces from Volunteers of Different Age Groups
Author(s) -
Hermie J. M. Harmsen,
A. C. M. Wildeboer-Veloo,
Jan Grijpstra,
Jan Knol,
John E. Degener,
Gjalt W. Welling
Publication year - 2000
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.66.10.4523-4527.2000
Subject(s) - enumeration , feces , 16s ribosomal rna , biology , human feces , ribosomal rna , microbiology and biotechnology , computational biology , bacteria , genetics , combinatorics , mathematics , gene
Two 16S rRNA-targeted probes were developed: one for theCoriobacterium group and the other for theAtopobium cluster (which comprises most of theCoriobacteriaceae species, including theCoriobacterium group). The new probes were based on sequences of three newCoriobacteriaceae strains isolated from human feces and clinical material and sequences from databases. Application of the probes to fecal samples showed that formula-fed infants had higher numbers ofCoriobacterium group cells in their feces than breast-fed infants. In addition, based on the presented results, it is hypothesized that with the increasing age of a person, the diversity ofAtopobium cluster species present in the feces increases.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom