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We used a quantitative PCR method targeting 16S ribosomal DNA using competitive PCR for specific detection of indigenousPseudomonas DNA in soil hot spots. The amount ofPseudomonas DNA corresponded to the number of culturablePseudomonas bacteria on Gould’s S1 agar. This represents the first use of PCR for quantification of indigenous bacteria in more than one sample of soil.

applied and environmental microbiology

Quantitative Selective PCR of 16S Ribosomal DNA Correlates Well with Selective Agar Plating in Describing Population Dynamics of Indigenous <i>Pseudomonas</i> spp. in Soil Hot Spots

Quantitative Selective PCR of 16S Ribosomal DNA Correlates Well with Selective Agar Plating in Describing Population Dynamics of Indigenous Pseudomonas spp. in Soil Hot Spots