Open AccessDirect detection of Listeria monocytogenes in 25 milliliters of raw milk by a two-step PCR with nested primers
Author(s) -
L M Herman,
J H De Block,
R. Moermans
Publication year - 1995
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.61.2.817-819.1995
Subject(s) - listeria monocytogenes , raw milk , nested polymerase chain reaction , listeria , biology , polymerase chain reaction , food science , microbiology and biotechnology , food microbiology , detection limit , bacteria , chemistry , chromatography , genetics , gene
A method for direct detection of Listeria monocytogenes in 25 ml of raw milk is presented. The detection limit can be situated between 10 and 5 CFU. The detection method is based on chemical extraction of the milk components and PCR amplification with two nested pairs of primers specific for Listeria monocytogenes.
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