Open AccessInhibition enzyme-linked immunosorbent assay for detection of Pseudomonas fluorescens on meat surfaces
Author(s) -
Patricia V. Eriksson,
G N di Paola,
Marcela F. Pasetti,
M.A. Manghi
Publication year - 1995
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.61.1.397-398.1995
Subject(s) - pseudomonas fluorescens , food spoilage , enumeration , microbiology and biotechnology , bacteria , salmonella , pseudomonas , pseudomonadaceae , contamination , pseudomonadales , enzyme , food science , enzyme assay , meat spoilage , biology , chemistry , biochemistry , genetics , combinatorics , mathematics , ecology
An inhibition enzyme-linked immunosorbent assay was developed for Pseudomonas fluorescens enumeration of meat surfaces. The assay detected contamination levels as low as 3 x 10(5) bacteria per ml and could be completed within 4 h. It could be used as a framework for a test system for quantifying P. fluorescens spoilage in meat products.
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