Open AccessA temperate phage of Streptomyces azureus
Author(s) -
Seiya Ogata,
Hikaru Suenaga,
Saki Hayashida
Publication year - 1985
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.49.1.201-204.1985
Subject(s) - temperateness , hindiii , lysogenic cycle , dna , restriction enzyme , cytosine , guanine , streptomyces , biology , bglii , bacteriophage , genetics , microbiology and biotechnology , escherichia coli , gene , bacteria , nucleotide
A new phage, SAt1, was isolated from soil on Streptomyces azureus ATCC 14921. This phage was able to lysogenize S. azureus. The percentage of lysogenic responses was ca. 10%. Electron microscopic observation showed that this phage belonged to group B of Bradley's morphological classification. The molecular mass of SAt1 DNA was ca. 24 megadaltons. The guanine-plus-cytosine content and the density of SAt1 DNA were ca. 71% and 1.724 g/cm3, respectively. A cleavage map of SAt1 DNA was constructed with restriction endonucleases BanIII, BglII, HindIII, and XbaI. Furthermore, some other characteristics of this phage were investigated.
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