Open AccessConversion of 2,4,6-trinitrophenol to a mutagen by Pseudomonas aeruginosa
Author(s) -
John F. Wyman,
Hannah E. Guard,
William D. Won,
Juliana Quay
Publication year - 1979
Publication title -
applied and environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.552
H-Index - 324
eISSN - 1070-6291
pISSN - 0099-2240
DOI - 10.1128/aem.37.2.222-226.1979
Subject(s) - picric acid , mutagen , histidine , pseudomonas aeruginosa , chemistry , salmonella , strain (injury) , biochemistry , amino acid substitution , pseudomonas , biology , microbiology and biotechnology , amino acid , mutation , bacteria , genetics , organic chemistry , dna , gene , anatomy
A strain of Pseudomonas aeruginosa reduced 2,4,6-trinitrophenol (picric acid) to 2-amino-4,6-dinitrophenol (picramic acid) under anaerobic conditions. Mutagenic assays of picric acid and picramic acid were carried out with histidine-requiring strains of Salmonella typhimurium. Picric acid (10 micrograms per plate) demonstrated mutagenicity (both frame shift and base substitution-gype mutations) only after activation with a rat liver homogenate preparation. Picramic acid (1 microgram per plate) induced both base pair substitution and frame shift-tupe mutations without activation by the rat liver preparation.
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