Open AccessComparative evaluation of isogenic mesodermal and ectomesodermal chondrocytes from human iPSCs for cartilage regeneration
Author(s) -
MingSong Lee,
Matthew J. Stebbins,
Hong-Li Jiao,
Hui-Ching Huang,
Ellen M. Leiferman,
Brian E. Walczak,
Sean P. Palecek,
Eric V. Shusta,
WanJu Li
Publication year - 2021
Publication title -
science advances
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.928
H-Index - 146
ISSN - 2375-2548
DOI - 10.1126/sciadv.abf0907
Subject(s) - induced pluripotent stem cell , microbiology and biotechnology , cartilage , hyaline cartilage , regeneration (biology) , biology , stem cell , chondrogenesis , anatomy , embryonic stem cell , pathology , osteoarthritis , articular cartilage , genetics , medicine , gene , alternative medicine
Generating phenotypic chondrocytes from pluripotent stem cells is of great interest in the field of cartilage regeneration. In this study, we differentiated human induced pluripotent stem cells into the mesodermal and ectomesodermal lineages to prepare isogenic mesodermal cell-derived chondrocytes (MC-Chs) and neural crest cell-derived chondrocytes (NCC-Chs), respectively, for comparative evaluation. Our results showed that both MC-Chs and NCC-Chs expressed hyaline cartilage-associated markers and were capable of generating hyaline cartilage-like tissue ectopically and at joint defects. Moreover, NCC-Chs revealed closer morphological and transcriptional similarities to native articular chondrocytes than MC-Chs. NCC-Ch implants induced by our growth factor mixture demonstrated increased matrix production and stiffness compared to MC-Ch implants. Our findings address how chondrocytes derived from pluripotent stem cells through mesodermal and ectomesodermal differentiation are different in activities and functions, providing the crucial information that helps make appropriate cell choices for effective regeneration of articular cartilage.