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Adult multipotent stromal cell cryopreservation: Pluses and pitfalls
Author(s) -
Duan Wei,
Lopez Mandi J.,
Hicok Kevin
Publication year - 2018
Publication title -
veterinary surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.652
H-Index - 79
eISSN - 1532-950X
pISSN - 0161-3499
DOI - 10.1111/vsu.12730
Subject(s) - cryopreservation , medicine , mesenchymal stem cell , stromal cell , cryoprotectant , regenerative medicine , multipotent stem cell , stem cell , bioinformatics , immunology , microbiology and biotechnology , pathology , biology , progenitor cell , embryo
Study and clinical testing of adult multipotent stromal cells (MSCs) are central to progressive improvements in veterinary regenerative medicine. Inherent limitations to long‐term culture preclude use for storage. Until cell line creation from primary isolates becomes routine, MSC stasis at cryogenic temperatures is required for this purpose. Many protocols and reagents, including cryoprotectants, used for veterinary MSCs are derived from those for human and rodent cells. Dissimilarities in cryopreservation strategies play a role in variable MSC behaviors. Familiarity with contemporary cryopreservation reagents and processes is essential to an appreciation of their impact on MSC survival and post‐cryopreservation behavior. In addition to these points, this review includes a brief history and description of current veterinary stem cell regulation.