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Effect of 7.5% hypertonic saline solution on whole blood coagulation in healthy dogs using thromboelastography
Author(s) -
Kim Hye young,
Nam Aryung,
Song Kun ho,
Youn Hwa young,
Seo Kyoung won
Publication year - 2020
Publication title -
journal of veterinary emergency and critical care
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.886
H-Index - 47
eISSN - 1476-4431
pISSN - 1479-3261
DOI - 10.1111/vec.12959
Subject(s) - thromboelastography , medicine , hypertonic saline , saline , coagulation , anesthesia , coagulation testing , tonicity , whole blood , dilution , hematocrit , physics , thermodynamics
Objective To evaluate the effects of 7.5% hypertonic saline solution (HSS) on whole blood coagulation in healthy dogs and to compare electrolyte and osmolality measurements between in vivo and in vitro dilution with HSS. Design Experimental study. Setting University teaching hospital. Animals Twelve adult purpose‐bred Beagles. Interventions All 12 dogs received 5 mL/kg 7.5% HSS at 1 mL/kg/min. After a 14‐day washout period, 5 of these dogs were randomly selected and received the same volume of 0.9% NaCl. Blood samples were collected before infusion, immediately after infusion, and at 30, 60, and 90 minutes after infusion for the measurement of coagulation using thromboelastography. For comparison of electrolyte concentrations and osmolality between in vitro dilution and in vivo dilution of HSS, 6‐mL blood samples were diluted with 7.5% HSS (1:18 ratio) at baseline. Measurements and Main Results None of the thromboelastography variables differed significantly between the 7.5% HSS group and the 0.9% NaCl group. The sodium and chloride levels, and the osmolality, were significantly increased at all postinfusion time points compared to baseline, while those levels were significantly higher with in vitro dilution than all postinfusion time points. However, almost all the values gradually decreased and became similar to baseline values in case of in vivo dilution. Conclusions The clinically relevant dose of 7.5% HSS (5 mL/kg) did not affect whole blood coagulation significantly in healthy Beagles. Further studies are necessary to assess the effect of HSS on blood coagulation in canine patients with shock.