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Background  Canine atopic dermatitis ( CAD ) is an inflammatory and pruritic allergic skin disease caused by interactions between genetic and environmental factors. Previously, a genome‐wide significant risk locus on canine chromosome 27 for  CAD  was identified in German shepherd dogs ( GSD s) and  Plakophilin‐2  (  PKP 2 ) was defined as the top candidate gene.  PKP 2 constitutes a crucial component of desmosomes and also is important in signalling, metabolic and transcriptional activities.    Objectives  The main objective was to evaluate the role of  PKP 2 in  CAD  by investigating  PKP 2 expression and desmosome structure in nonlesional skin from  CAD ‐affected (carrying the top  GWAS SNP  risk allele) and healthy  GSD s. We also aimed at defining the cell types in the skin that express  PKP 2 and its intracellular location.    Animals/Methods  Skin biopsies were collected from nine  CAD ‐affected and five control  GSD s. The biopsies were frozen for immunofluorescence and fixed for electron microscopy immunolabelling and morphology.    Results  We observed the novel finding of  PKP 2 expression in dendritic cells and T cells in dog skin. Moreover, we detected that  PKP 2 was more evenly expressed within keratinocytes compared to its desmosomal binding‐partner plakoglobin.  PKP 2 protein was located in the nucleus and on keratin filaments attached to desmosomes. No difference in  PKP 2 abundance between  CAD  cases and controls was observed.    Conclusion  Plakophilin‐2 protein in dog skin is expressed in both epithelial and immune cells; based on its subcellular location its functional role is implicated in both nuclear and structural processes.

veterinary dermatology

Comparison of cellular location and expression of Plakophilin‐2 in epidermal cells from nonlesional atopic skin and healthy skin in German shepherd dogs