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Fluorogen activating protein toolset for protein trafficking measurements
Author(s) -
Perkins Lydia A.,
Bruchez Marcel P.
Publication year - 2020
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1111/tra.12722
Subject(s) - biology , transport protein , computational biology , microbiology and biotechnology , protein subcellular localization prediction , nanotechnology , biochemistry , gene , materials science
Assessing protein trafficking in live cells is a core strength of fluorogen activating protein technology. The rapid and specific labeling of a tagged protein of interest, intracellular or surface, with a variety of fluorogenic dye derivatives creates a toolset suitable for scalable, dynamic multi‐color fluorescence experiments and direct quantitative trafficking‐related measurements in single cells and cell populations, using fluorometry, flow cytometry and microscopy.