English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Open

The  Drosophila  type I transmembrane protein Crumbs is an apical determinant required for the maintenance of apico‐basal epithelial cell polarity. The level of Crumbs at the plasma membrane is crucial, but how it is regulated is poorly understood. In a genetic screen for regulators of Crumbs protein trafficking we identified Sar1, the core component of the coat protein complex  II  transport vesicles.  sar1  mutant embryos show a reduced plasma membrane localization of Crumbs, a defect similar to that observed in  haunted  and  ghost  mutant embryos, which lack Sec23 and  Sec24CD , respectively. By pulse‐chase assays in  Drosophila  Schneider cells and analysis of protein transport kinetics based on Endoglycosidase H resistance we identified an  RNKR  motif in Crumbs, which contributes to efficient  ER  export. The motif identified fits the highly conserved di‐basic  RxKR  motif and mediates interaction with Sar1. The  RNKR  motif is also required for plasma membrane delivery of transgene‐encoded Crumbs in epithelial cells of  Drosophila  embryos. Our data are the first to show that a di‐basic motif acts as a signal for  ER  exit of a type I plasma membrane protein in a metazoan organism.

A Conserved Di‐Basic Motif of Drosophila Crumbs Contributes to Efficient ER Export