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Cadaverine regulates biotin synthesis to modulate primary root growth in Arabidopsis
Author(s) -
Gibbs Nicole M.,
Su ShihHeng,
LopezNieves Samuel,
Mann Stéphane,
Alban Claude,
Maeda Hiroshi A.,
Masson Patrick H.
Publication year - 2021
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.15417
Subject(s) - cadaverine , biology , putrescine , biochemistry , biotin , biosynthesis , arabidopsis , spermine , polyamine , enzyme , gene , mutant
SUMMARY Cadaverine, a polyamine, has been linked to modification of root growth architecture and response to environmental stresses in plants. However, the molecular mechanisms that govern the regulation of root growth by cadaverine are largely unexplored. Here we conducted a forward genetic screen and isolated a mutation, cadaverine hypersensitive 3 ( cdh3 ), which resulted in increased root‐growth sensitivity to cadaverine, but not other polyamines. This mutation affects the BIO3‐BIO1 biotin biosynthesis gene. Exogenous supply of biotin and a pathway intermediate downstream of BIO1, 7,8‐diaminopelargonic acid, suppressed this cadaverine sensitivity phenotype. An in vitro enzyme assay showed cadaverine inhibits the BIO3‐BIO1 activity. Furthermore, cadaverine‐treated seedlings displayed reduced biotinylation of Biotin Carboxyl Carrier Protein 1 of the acetyl‐coenzyme A carboxylase complex involved in de novo fatty acid biosynthesis, resulting in decreased accumulation of triacylglycerides. Taken together, these results revealed an unexpected role of cadaverine in the regulation of biotin biosynthesis, which leads to modulation of primary root growth of plants.