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Arabidopsis acyl‐ C o A ‐binding protein ACBP 3 participates in plant response to hypoxia by modulating very‐long‐chain fatty acid metabolism
Author(s) -
Xie LiJuan,
Yu LuJun,
Chen QinFang,
Wang FengZhu,
Huang Li,
Xia FanNv,
Zhu TianRen,
Wu JianXin,
Yin Jian,
Liao Bin,
Yao Nan,
Shu Wensheng,
Xiao Shi
Publication year - 2015
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1111/tpj.12692
Subject(s) - biology , microscale thermophoresis , biochemistry , mutant , phosphatidylserine , transcription factor , oxylipin , arabidopsis , microbiology and biotechnology , gene , phospholipid , membrane
Summary In A rabidopsis thaliana , acyl‐CoA‐binding proteins ( ACBP s) are encoded by a family of six genes ( ACBP 1 to ACBP 6 ), and are essential for diverse cellular activities. Recent investigations suggest that the membrane‐anchored ACBP s are involved in oxygen sensing by sequestration of group VII ethylene‐responsive factors under normoxia. Here, we demonstrate the involvement of A rabidopsis ACBP 3 in hypoxic tolerance. ACBP 3 transcription was remarkably induced following submergence under both dark ( DS ) and light ( LS ) conditions. ACBP 3 ‐overexpressors ( ACBP 3‐ OE s ) showed hypersensitivity to DS , LS and ethanolic stresses, with reduced transcription of hypoxia‐responsive genes as well as accumulation of hydrogen peroxide in the rosettes. In contrast, suppression of ACBP 3 in ACBP 3‐ KO s enhanced plant tolerance to DS , LS and ethanol treatments. By analyses of double combinations of OE ‐1 with npr1‐5 , coi1‐2 , ein3‐1 as well as ctr1‐1 mutants, we observed that the attenuated hypoxic tolerance in ACBP 3‐ OE s was dependent on NPR 1‐ and CTR 1‐mediated signaling pathways. Lipid profiling revealed that both the total amounts and very‐long‐chain species of phosphatidylserine (C42:2‐ and C42:3‐ PS ) and glucosylinositolphosphorylceramides (C22:0‐, C22:1‐, C24:0‐, C24:1‐, and C26:1‐ GIPC ) were significantly lower in ACBP 3‐ OE s but increased in ACBP 3‐ KO s upon LS exposure. By microscale thermophoresis analysis, the recombinant ACBP 3 protein bound VLC acyl‐CoA esters with high affinities in vitro . Further, a knockout mutant of MYB 30, a master regulator of very‐long‐chain fatty acid ( VLCFA ) biosynthesis, exhibited enhanced sensitivities to LS and ethanolic stresses, phenotypes that were ameliorated by ACBP 3‐ RNA i . Taken together, these findings suggest that A rabidopsis ACBP 3 participates in plant response to hypoxia by modulating VLCFA metabolism.