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Detection of 549 new HLA alleles in potential stem cell donors from the United States, Poland and Germany
Author(s) -
HernándezFrederick C. J.,
Cereb N.,
Giani A. S.,
Ruppel J.,
Maraszek A.,
Pingel J.,
Sauter J.,
Schmidt A. H.,
Yang S. Y.
Publication year - 2016
Publication title -
hla
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.347
H-Index - 99
eISSN - 2059-2310
pISSN - 2059-2302
DOI - 10.1111/tan.12721
Subject(s) - allele , human leukocyte antigen , nonsynonymous substitution , genetics , biology , homologous chromosome , null allele , hla a , histocompatibility testing , hla c , gene , antigen , genome
We characterized 549 new human leukocyte antigen ( HLA ) class I and class II alleles found in newly registered stem cell donors as a result of high‐throughput HLA typing. New alleles include 101 HLA ‐A, 132 HLA ‐B, 105 HLA ‐C, 2 HLA‐DRB1 , 89 HLA‐DQB1 and 120 HLA‐DPB1 alleles. Mainly, new alleles comprised single nucleotide variations when compared with homologous sequences. We identified nonsynonymous nucleotide mutations in 70.7% of all new alleles, synonymous variations in 26.4% and nonsense substitutions in 2.9% (null alleles). Some new alleles (55, 10.0%) were found multiple times, HLA‐DPB1 alleles being the most frequent among these. Furthermore, as several new alleles were identified in individuals from ethnic minority groups, the relevance of recruiting donors belonging to such groups and the importance of ethnicity data collection in donor centers and registries is highlighted.