English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Open

The homeostatic control of the redox system (the redoxome) in mammalian cells depends upon a large number of interacting molecules, which tend to buffer the electronegativity of cells against oxidants or reductants. Some of these components kill – at high concentration – microbes and by‐stander normal cells, elaborated by professional phagocytes. We examined whether a simple,  in vitro  chemiluminescence set‐up, utilizing redox components from human polymorphonuclear neutrophils ( PMN ) and red blood cells ( RBC ), could clarify some unexplained workings of the redoxome.  PMN  or purified myeloperoxidase ( MPO ) triggers formation of reactive oxygen species ( ROS ), quantified by light emission from oxidized luminol. Both  PMN  and  RBC  can generate abundant amounts of  ROS , necessitating the presence of a high‐capacity redoxome to keep the cellular electronegativity within physiological limits. We obtained proof‐of‐principle evidence that our assay could assess redox effects, but also demonstrated the intricacies of redox reactions. Simple dose–responses were found, as for the  PMN  proteins  S 100 A 9 ( A 9) and  S 100 A 8 ( A 8), and the system also revealed the reducing capacity of vitamin  B 12 ( C bl) and lutein. However, increased concentrations of oxidants in the assay mixture could decrease the chemiluminescence. Even more remarkable,  A 9 and   NaOCl   together stimulated the  MPO  response, but alone they inhibited  MPO  chemiluminescence. Biphasic responses were also recorded for some dose–response set‐ups and are tentatively explained by a ‘balance hypothesis’ for the redoxome.

Intricacies of Redoxome Function Demonstrated with a Simple In vitro Chemiluminescence Method, with Special Reference to Vitamin B12 as Antioxidant