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Gibberellin biosynthesis and signal transduction is essential for internode elongation in deepwater rice
Author(s) -
AYANO MADOKA,
KANI TAKAHIRO,
KOJIMA MIKIKO,
SAKAKIBARA HITOSHI,
KITAOKA TAKUYA,
KUROHA TAKESHI,
ANGELESSHIM ROSALYN B.,
KITANO HIDEMI,
NAGAI KEISUKE,
ASHIKARI MOTOYUKI
Publication year - 2014
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.12377
Subject(s) - elongation , plant stem , gibberellin , biology , botany , chemistry , ultimate tensile strength , materials science , metallurgy
Under flooded conditions, the leaves and internodes of deepwater rice can elongate above the water surface to capture oxygen and prevent drowning. Our previous studies showed that three major quantitative trait loci ( QTL ) regulate deepwater‐dependent internode elongation in deepwater rice. In this study, we investigated the age‐dependent internode elongation in deepwater rice. We also investigated the relationship between deepwater‐dependent internode elongation and the phytohormone gibberellin ( GA ) by physiological and genetic approach using a QTL pyramiding line ( NIL ‐1 + 3 + 12). Deepwater rice did not show internode elongation before the sixth leaf stage under deepwater condition. Additionally, deepwater‐dependent internode elongation occurred on the sixth and seventh internodes during the sixth leaf stage. These results indicate that deepwater rice could not start internode elongation until the sixth leaf stage. U ltra‐performance liquid chromatography tandem mass‐spectrometry ( UPLC ‐ MS / MS ) method for the phytohormone contents showed a deepwater‐dependent GA 1 and GA 4 accumulation in deepwater rice. Additionally, a GA inhibitor abolished deepwater‐dependent internode elongation in deepwater rice. On the contrary, GA feeding mimicked internode elongation under ordinary growth conditions. However, mutations in GA biosynthesis and signal transduction genes blocked deepwater‐dependent internode elongation. These data suggested that GA biosynthesis and signal transduction are essential for deepwater‐dependent internode elongation in deepwater rice.