English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Open

Summary  MiRNAs are important posttranscriptional regulators of plant development. Many miRNAs, such as the conserved miR164 species, are encoded by families of  MIRNA  genes, but the specific roles of individual  MIRNA  genes are largely undefined. Here, we characterize the functions and regulatory mechanisms of  SlMIR164A , one of the primary genes of Sly‐miR164, in tomato. We show that  SlMIR164A  is preferentially expressed at late stages of fruit development and plays a vital role in controlling fruit ripening and quality. Loss of function of  SlMIR164A  by CRISPR/Cas9‐mediated mutagenesis results in accelerated fruit ripening and enhanced chloroplast development, which leads to altered sugar and organic acid contents and affects the nutritional quality of fruits. We also show that  SlMIR164A  modulates fruit ripening and quality through specific target genes,  SlNAM2  and  SlNAM3 , which control key regulators of chloroplast function and fruit ripening processes.  MIR164  genes have been shown to play conserved roles in regulating organ ageing, such as leaf senescence and fruit ripening, in a variety of plants, but whether and how their family members in tomato exert the same function remain to be elucidated. Our results reveal a previously undiscovered role of  SlMIR164A  in ripening control, which will further our understanding of the actions of  MIR164  family, as well as the mechanisms of fruit ripening and quality control in tomato. Moreover, as loss of  SlMIR164A  exhibits minor impacts on organ morphology, our results can be leveraged in tomato breeding for specific manipulation of fruit ripening and quality to facilitate tomato improvement in agriculture.

SlMIR164A regulates fruit ripening and quality by controlling SlNAM2 and SlNAM3 in tomato