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Summary  Seed storage proteins (SSPs) are determinants of wheat end‐product quality. SSP synthesis is mainly regulated at the transcriptional level. Few transcriptional regulators of SSP synthesis have been identified in wheat and this study aims to identify novel SSP gene regulators. Here, the R2R3 MYB transcription factor  TuODORANT1  from  Triticum urartu  was found to be preferentially expressed in the developing endosperm during grain filling. In common wheat ( Triticum aestivum ) overexpressing  TuODORANT1 , the transcription levels of all the SSP genes tested by RNA‐Seq analysis were reduced by 49.71% throughout grain filling, which contributed to 13.38%–35.60% declines in the total SSP levels of mature grains. In  in vitro  assays,  TuODORANT1  inhibited both the promoter activities and the transcription of SSP genes by 1‐ to 13‐fold. The electrophoretic mobility shift assay (EMSA) and ChIP‐qPCR analysis demonstrated that TuODORANT1 bound to the  cis ‐elements 5′‐T/CAACCA‐3′ and 5′‐T/CAACT/AG‐3′ in SSP gene promoters both  in vitro  and  in vivo . Similarly, the homolog  TaODORANT1  in common wheat hindered both the promoter activities and the transcription of SSP genes by 1‐ to 112‐fold  in vitro . Knockdown of  TaODORANT1  in common wheat led to 14.73%–232.78% increases in the transcription of the tested SSP genes, which contributed to 11.43%–19.35% elevation in the total SSP levels. Our data show that both  TuODORANT1  and  TaODORANT1  are repressors of SSP synthesis.

plant biotechnology journal

The MYB family transcription factor  TuODORANT1  from  Triticum urartu  and the homolog  TaODORANT1  from  Triticum aestivum  inhibit seed storage protein synthesis in wheat