English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Open

Summary  MicroRNAs (miRNAs) are small non‐coding RNAs that direct post‐transcriptional gene silencing in plant development and stress responses through cleavage or translational repression of target mRNAs. Here, we report the identification and functional characterization of a new member of the miR812 family in rice (named as miR812w) involved in disease resistance. miR812w is present in cultivated  Oryza  species, both  japonica  and  indica  subspecies, and wild rice species within the  Oryza  genus, but not in dicotyledonous species. miR812w is a 24nt‐long that requires DCL3 for its biogenesis and is loaded into AGO4 proteins. Whereas overexpression of miR812w increased resistance to infection by the rice blast fungus  Magnaporthe oryzae , CRISPR/Cas9‐mediated  MIR812w  editing enhances disease susceptibility, supporting that miR812w plays a role in blast resistance. We show that miR812w derives from the  Stowaway  type of rice MITEs (Miniature Inverted‐Repeat Transposable Elements). Moreover, miR812w directs DNA methylation  in trans  at target genes that have integrated a  Stowaway  MITE copy into their 3′ or 5′ untranslated region ( ACO3 ,  CIPK10, LRR  genes), as well as  in cis  at the  MIR812w  locus. The target genes of miR812 were found to be hypo‐methylated around the miR812 recognition site, their expression being up‐regulated in transgene‐free CRISPR/Cas9‐edited miR812 plants. These findings further support that, in addition to post‐transcriptional regulation of gene expression, miRNAs can exert their regulatory function at the transcriptional level. This relationship between miR812w and Stowaway MITEs integrated into multiple coding genes might eventually create a network for miR812w‐mediated regulation of gene expression with implications in rice immunity.

A novel Transposable element‐derived microRNA participates in plant immunity to rice blast disease