English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Open

Summary  The development of embryo sacs is crucial for seed production in plants, but the genetic basis regulating the meiotic crossover formation in the macrospore and microspore mother cells remains largely unclear. Here, we report the characterization of a spontaneous rice  female sterile variation 1  mutant ( fsv1 ) that showed severe embryo sacs abortion with low seed‐setting rate. Through map‐based cloning and functional analyses, we isolated the causal gene of  fsv1 ,  OsMLH3  encoding a MutL‐homolog 3 protein, an ortholog of HvMLH3 in barley and AtMLH3 in  Arabidopsis . OsMLH3 and OsMLH1 (MutL‐homolog 1) interact to form a heterodimer (MutLγ) to promote crossover formation in the macrospore and microspore mother cells and development of functional megaspore during meiosis, defective OsMLH3 or OsMLH1 in  fsv1  and CRISPR/Cas9‐based knockout lines results in reduced type I crossover and bivalent frequency. The  fsv1  and  OsMLH3 ‐knockout lines are valuable germplasms for development of female sterile restorer lines for mechanized seed production of hybrid rice.

Rice MutLγ, the MLH1–MLH3 heterodimer, participates in the formation of type I crossovers and regulation of embryo sac fertility