Open AccessQ Dtbn1 , an F‐box gene affecting maize tassel branch number by a dominant model
Author(s) -
Qin Xiner,
Tian Shike,
Zhang Wenliang,
Dong Xue,
Ma Chengxin,
Wang Yi,
Yan Jianbing,
Yue Bing
Publication year - 2021
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/pbi.13540
Subject(s) - tassel , biology , gene , candidate gene , genetics , population , quantitative trait locus , single nucleotide polymorphism , zea mays , genotype , agronomy , demography , sociology
Summary Tassel branch number (TBN) is one of the important agronomic traits that directly contribute to grain yield in maize ( Zea mays L.), and identification of genes precisely regulating TBN in the parental lines is important for maize hybrid breeding. In this study, a quantitative trait nucleotide (QTN), Q Dtbn1 , related to tassel branch number was identified using a testcrossing association mapping population through association mapping with the Indels/SNPs in the 5′‐UTR (untranslated region) of Zm00001d053358 , which encodes a Kelch repeat‐containing F‐box protein. Q Dtbn1 was further confirmed to be associated with TBN by a dominant model using an F 2 population, and over‐expressing of the candidate gene resulted in a decreasing of TBN, implying that Q Dtbn1 was governed by the candidate gene with a negative model. This makes Q Dtbn1 very useful in maize hybrid breeding. Q Dtbn1 could interact with a maize Skp1‐like protein and a SnRK1 protein, and the SnRK1 could also interact with a SnRK2.8 protein. In addition, quantitative real‐time PCR assay showed that five substrates of SnRK2 were down‐regulated in the over‐expressed plants. These imply that the SCF (Skp1/Cul1/F‐box protein/Roc1) complex and ABA signal pathway might be involved in the modulation of TBN in maize.