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Summary  The LRK10‐like receptor kinases (LRK10L‐RLKs) are ubiquitously present in higher plants, but knowledge of their expression and function is still limited. Here, we report expression and functional analysis of TtdLRK10L‐1, a typical LRK10L‐RLK in durum wheat ( Triticum turgidum  L. ssp.  durum ). The introns of  TtdLRK10L‐1  contained multiple kinds of predicted  cis ‐elements. To investigate the potential effect of these  cis ‐elements on TtdLRK10L‐1 expression and function, two types of transgenic wheat lines were prepared, which expressed a GFP‐tagged TtdLRK10L‐1 protein (TtdLRK10L‐1:GFP) from the cDNA or genomic DNA (gDNA) sequence of  TtdLRK10L‐1  under the native promoter. TtdLRK10L‐1:GFP expression was up‐regulated by the powdery mildew pathogen  Blumeria graminis  f. sp.  tritici  ( Bgt ) in both types of transgenic plants, with the scale of the elevation being much stronger in the gDNA lines. Both types of transgenic plants exhibited enhanced resistance to  Bgt  infection relative to wild type control. Notably, the  Bgt  defence activated in the gDNA lines was significantly stronger than that in the cDNA lines. Further analysis revealed that a putative MYB transcription factor binding site (MYB‐BS, CAGTTA) located in  TtdLRK10L‐1  intron I was critical for the efficient expression and function of TtdLRK10L‐1 in  Bgt  defence. This MYB‐BS could also increase the activity of a superpromoter widely used in ectopic gene expression studies in plants. Together, our results deepen the understanding of the expression and functional characteristics of LRK10L‐RLKs. TtdLRK10L‐1 is likely useful for further dissecting the molecular processes underlying wheat defence against  Bgt  and for developing  Bgt  resistant wheat crops.

Efficient expression and function of a receptor‐like kinase in wheat powdery mildew defence require an intron‐located MYB binding site