Open AccessEfficient expression and function of a receptor‐like kinase in wheat powdery mildew defence require an intron‐located MYB binding site
Author(s) -
Xia Tengfei,
Yang Yanping,
Zheng Hongyuan,
Han Xinyun,
Jin Huaibing,
Xiong Zijun,
Qian Weiqiang,
Xia Lanqi,
Ji Xiang,
Li Guangwei,
Wang Daowen,
Zhang Kunpu
Publication year - 2021
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/pbi.13512
Subject(s) - biology , blumeria graminis , myb , powdery mildew , ectopic expression , genetics , intron , gene , complementary dna , gene expression , genetically modified rice , transgene , genetically modified crops , promoter , gene silencing , botany , plant disease resistance
Summary The LRK10‐like receptor kinases (LRK10L‐RLKs) are ubiquitously present in higher plants, but knowledge of their expression and function is still limited. Here, we report expression and functional analysis of TtdLRK10L‐1, a typical LRK10L‐RLK in durum wheat ( Triticum turgidum L. ssp. durum ). The introns of TtdLRK10L‐1 contained multiple kinds of predicted cis ‐elements. To investigate the potential effect of these cis ‐elements on TtdLRK10L‐1 expression and function, two types of transgenic wheat lines were prepared, which expressed a GFP‐tagged TtdLRK10L‐1 protein (TtdLRK10L‐1:GFP) from the cDNA or genomic DNA (gDNA) sequence of TtdLRK10L‐1 under the native promoter. TtdLRK10L‐1:GFP expression was up‐regulated by the powdery mildew pathogen Blumeria graminis f. sp. tritici ( Bgt ) in both types of transgenic plants, with the scale of the elevation being much stronger in the gDNA lines. Both types of transgenic plants exhibited enhanced resistance to Bgt infection relative to wild type control. Notably, the Bgt defence activated in the gDNA lines was significantly stronger than that in the cDNA lines. Further analysis revealed that a putative MYB transcription factor binding site (MYB‐BS, CAGTTA) located in TtdLRK10L‐1 intron I was critical for the efficient expression and function of TtdLRK10L‐1 in Bgt defence. This MYB‐BS could also increase the activity of a superpromoter widely used in ectopic gene expression studies in plants. Together, our results deepen the understanding of the expression and functional characteristics of LRK10L‐RLKs. TtdLRK10L‐1 is likely useful for further dissecting the molecular processes underlying wheat defence against Bgt and for developing Bgt resistant wheat crops.