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Summary   Brassica napus  is highly susceptible towards  Verticillium longisporum  ( Vl43 ) with no effective genetic resistance. It is believed that the fungus reprogrammes plant physiological processes by up‐regulation of so‐called susceptibility factors to establish a compatible interaction. By transcriptome analysis, we identified genes, which were activated/up‐regulated in rapeseed after  Vl43  infection. To test whether one of these genes is functionally involved in the infection process and loss of function would lead to decreased susceptibility, we firstly challenged KO lines of corresponding Arabidopsis orthologs with  Vl43  and compared them with wild‐type plants. Here, we report that the KO of  AtCRT1a  results in drastically reduced susceptibility of plants to  Vl43 . To prove  crt1a  mutation also decreases susceptibility in  B. napus , we identified 10 mutations in a TILLING population. Three T3 mutants displayed increased resistance as compared to the wild type. To validate the results, we generated CRISPR/Cas‐induced  BnCRT1a  mutants, challenged T2 plants with  Vl43  and observed an overall reduced susceptibility in 3 out of 4 independent lines. Genotyping by allele‐specific sequencing suggests a major effect of mutations in the  CRT1a  A‐genome copy, while the C‐genome copy appears to have no significant impact on plant susceptibility when challenged with  Vl43 . As revealed by transcript analysis, the loss of function of  CRT1a  results in activation of the ethylene signalling pathway, which may contribute to reduced susceptibility. Furthermore, this study demonstrates a novel strategy with great potential to improve plant disease resistance.

plant biotechnology journal

Loss of function of CRT1a (calreticulin) reduces plant susceptibility to  Verticillium longisporum  in both  Arabidopsis thaliana  and oilseed rape ( Brassica napus )