Open AccessMetabolic rewiring of synthetic pyruvate dehydrogenase bypasses for acetone production in cyanobacteria
Author(s) -
Lee Hyun Jeong,
Son Jigyeong,
Sim Sang Jun,
Woo Han Min
Publication year - 2020
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/pbi.13342
Subject(s) - cyanobacteria , carbon fixation , photosynthesis , pyruvate dehydrogenase complex , acetone , biology , biochemistry , assimilation (phonology) , acetyl coa , metabolic engineering , enzyme , bacteria , linguistics , genetics , philosophy
Summary Designing synthetic pathways for efficient CO 2 fixation and conversion is essential for sustainable chemical production. Here we have designed a synthetic acetate‐acetyl‐CoA/malonyl‐CoA (AAM) bypass to overcome an enzymatic activity of pyruvate dehydrogenase complex. This synthetic pathway utilizes acetate assimilation and carbon rearrangements using a methyl malonyl‐CoA carboxyltransferase. We demonstrated direct conversion of CO 2 into acetyl‐CoA‐derived acetone as an example in photosynthetic Synechococcus elongatus PCC 7942 by increasing the acetyl‐CoA pools. The engineered cyanobacterial strain with the AAM‐bypass produced 0.41 g/L of acetone at 0.71 m/day of molar productivity. This work clearly shows that the synthetic pyruvate dehydrogenase bypass (AAM‐bypass) is a key factor for the high‐level production of an acetyl‐CoA‐derived chemical in photosynthetic organisms.