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Summary  Sclerotinia stem rot (SSR) caused by  Sclerotinia sclerotiorum  is a devastating disease of rapeseed ( Brassica napus  L.). To date, the genetic mechanisms of rapeseed’ interactions with  S. sclerotiorum  are not fully understood, and molecular‐based breeding is still the most effective control strategy for this disease. Here,  Arabidopsis thaliana GDSL1  was characterized as an extracellular GDSL lipase gene functioning in  Sclerotinia  resistance. Loss of  AtGDSL1  function resulted in enhanced susceptibility to  S. sclerotiorum . Conversely, overexpression of  AtGDSL1  in  B. napus  enhanced resistance, which was associated with increased reactive oxygen species (ROS) and salicylic acid (SA) levels, and reduced jasmonic acid levels. In addition,  AtGDSL1  can cause an increase in lipid precursor phosphatidic acid levels, which may lead to the activation of downstream ROS/SA defence‐related pathways. However, the rapeseed  BnGDSL1  with highest sequence similarity to  AtGDSL1  had no effect on SSR resistance. A candidate gene association study revealed that only one  AtGDSL1  homolog from rapeseed,  BnaC07g35650D  ( BnGLIP1 ), significantly contributed to resistance traits in a natural  B. napus  population, and the resistance function was also confirmed by a transient expression assay in tobacco leaves. Moreover, genomic analyses revealed that  BnGLIP1  locus was embedded in a selected region associated with SSR resistance during the breeding process, and its elite allele type belonged to a minor allele in the population. Thus,  BnGLIP1  is the functional equivalent of  AtGDSL1  and has a broad application in rapeseed  S. sclerotiorum ‐resistance breeding.

plant biotechnology journal

Arabidopsis GDSL1  overexpression enhances rapeseed  Sclerotinia sclerotiorum  resistance and the functional identification of its homolog in  Brassica napus