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Summary  Boron (B) deficiency is one of the major causes of growth inhibition and yield reduction in  Brassica napus  ( B. napus ). However, the molecular mechanisms of low B adaptation in  B. napus  are largely unknown. Here, fifty‐one BnaWRKY transcription factors were identified as responsive to B deficiency in  B. napus , in which BnaAn.WRKY26, BnaA9.WRKY47, BnaA1.WKRY53 and BnaCn.WRKY57 were tested in yeast one‐hybrid assays and showed strong binding activity with conserved sequences containing a W box in the promoters of the B transport‐related genes  BnaNIP5;1s  and  BnaBOR1s . Green fluorescent protein fused to the target protein demonstrated the nuclear localization of BnaA9.WRKY47. CRISPR/Cas9‐mediated knockout lines of  BnaA9.WRKY47  in  B. napus  had increased sensitivity to low B and lower contents of B than wild‐type plants. In contrast, overexpression of  BnaA9.WRKY47  enhanced the adaptation to low B with higher B contents in tissues than in wild‐type plants. Consistent with the phenotypic response and B accumulation in these transgenic lines, the transcription activity of  BnaA3.NIP5;1,  a B efficiency candidate gene, was decreased in the knockout lines but was significantly increased in the overexpressing lines under low B conditions. Electrophoretic mobility shift assays, transient expression experiments in tobacco and in situ hybridizations showed that BnaA9.WRKY47 directly activated  BnaA3.NIP5;1  expression through binding to the specific  cis ‐element. Taken together, our findings support BnaWRKYs as new participants in response to low B, and BnaA9.WRKY47 contributes to the adaptation of  B. napus  to B deficiency through up‐regulating  BnaA3.NIP5;1  expression to facilitate efficient B uptake.

plant biotechnology journal

Transcription factor BnaA9.WRKY47 contributes to the adaptation of  Brassica napus  to low boron stress by up‐regulating the boric acid channel gene  BnaA3.NIP5;1