Open AccessHeterologous expression of chloroplast‐localized geranylgeranyl pyrophosphate synthase confers fast plant growth, early flowering and increased seed yield
Author(s) -
Tata Sandeep Kumar,
Jung Jihye,
Kim YoonHa,
Choi Jun Young,
Jung JiYul,
Lee InJung,
Shin Jeong Sheop,
Ryu Stephen Beungtae
Publication year - 2016
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/pbi.12333
Subject(s) - biology , gibberellin , geranylgeranyl pyrophosphate , shoot , botany , nicotiana tabacum , genetically modified crops , helianthus annuus , gibberellic acid , germination , horticulture , transgene , sunflower , gene , biochemistry , prenylation , enzyme
Summary Geranylgeranyl pyrophosphate synthase ( GGPS ) is a key enzyme for a structurally diverse class of isoprenoid biosynthetic metabolites including gibberellins, carotenoids, chlorophylls and rubber. We expressed a chloroplast‐targeted GGPS isolated from sunflower ( Helianthus annuus ) under control of the cauliflower mosaic virus 35S promoter in tobacco ( Nicotiana tabacum ). The resulting transgenic tobacco plants expressing heterologous GGPS showed remarkably enhanced growth (an increase in shoot and root biomass and height), early flowering, increased number of seed pods and greater seed yield compared with that of GUS ‐transgenic lines (control) or wild‐type plants. The gibberellin levels in Ha GGPS ‐transgenic plants were higher than those in control plants, indicating that the observed phenotype may result from increased gibberellin content. However, in Ha GGPS ‐transformant tobacco plants, we did not observe the phenotypic defects such as reduced chlorophyll content and greater petiole and stalk length, which were previously reported for transgenic plants expressing gibberellin biosynthetic genes. Fast plant growth was also observed in Ha GGPS ‐expressing Arabidopsis and dandelion plants. The results of this study suggest that GGPS expression in crop plants may yield desirable agronomic traits, including enhanced growth of shoots and roots, early flowering, greater numbers of seed pods and/or higher seed yield. This research has potential applications for fast production of plant biomass that provides commercially valuable biomaterials or bioenergy.